| dc.description.abstract | Cyclamate is one of the sweeteners synthetic that is often used to the
various types of food products. Determining the concentration of cyclamate in
energy drinks with registration number POMSD152246XXX has been
performed using High Performance Liquid Chromatography (HPLC) with aim
to knowing accordance concentration of cyclamates in the sample with the
requirements according to the decree of head of BPOM 2004.
In this analysis, HPLC is used reversed-phase column with a stationary
phase coloum C18, particle size 10 μm (3,9 x 300 nm), mobile phase of buffer
phospate pH 3,5 : acetonitrile (80:20), UV-Vis detector at 195 nm wavelenght
measurements. Separation of cyclamate through isocratic elution technicue with
a flow rate of 1 ml/min and 20 μl injection volume.
The result showed the value of coefficient correlation (r) 0,9999; limit of
detection (LOD) 2,22 x 10 -3 g/kg; limit of quantitation (LOQ) 7,399 x 10-3
g/kg. Determining concentration of cyclamates in sample is 0,47 ± 0,058 g/kg
ingredients. That is not exceed the requirements according to the decree of head
of BPOM 2004, which is 1,25 g/kg ingredients. | en_US |
