| dc.contributor.advisor | RAHMAWATI, ATIEK DRIANA | |
| dc.contributor.author | PRATIWI, INDAH SANTI | |
| dc.date.accessioned | 2018-10-15T07:22:38Z | |
| dc.date.available | 2018-10-15T07:22:38Z | |
| dc.date.issued | 2018-08-24 | |
| dc.identifier.uri | http://repository.umy.ac.id/handle/123456789/22103 | |
| dc.description | Latar Belakang : Penyakit gigi dan mulut menduduki urutan pertama yaitu sebesar
60% di Indonesia. Salah satu bakteri penyebab penyakit rongga mulut adalah bakteri
Staphylococcus aureus. Bakteri Staphylococcus aureus merupakan penyebab gingivitis
yang termasuk dalam golongan bakteri gram positif, anaerob. Nanas (Ananas comosus)
adalah tanaman yang telah tersebar luas ke seluruh dunia dan dapat tumbuh di dataran
rendah maupun dataran tinggi. Pada bagian kulit buah nanas (Ananas comosus)
mengandung zat-zat aktif yang memiliki daya antibakteri.
Tujuan : penelitian ini bertujuan untuk menguji Kadar Hambat Minimal (KHM) dan
Kadar Bunuh Minimal (KBM) ekstrak kulit nanas (Ananas comosus) dengan
konsentrasi 100%, 50%, 25%, 12,5%, 6,25%, 3,125%, 1,56%, dan 0,78% terhadap
bakteri Staphylococcus aureus secara in vitro.
Metode : Jenis penelitian adalah penelitian ekperimental murni laboratoris (in vitro).
Uji daya antibakteri dilakukan dengan metode dilusi cair pada media Brain Heart
Infusion (BHI) dan metode dilusi padat pada media Triton Soya Agar (TSA). Ekstrak
kulit nanas (Ananas comosus) diencerkan dengan cara berseri kedalam beberapa
konsentrasi: 100%, 50%, 25%, 12,5%, 6,25%, 3,125%, 1,56%, dan 0,78%. Kadar
hambat minimal dan kadar bunuh minimal ditentukan dengan mengamati dari
pertumbuhan bakteri Staphylococcus aureus media BHI dan TSA.
Hasil : Kadar Hambat Minimal (KHM) terdapat pada konsentrasi 1,56% dan Kadar
Bunuh minimal (KBM) terdapat pada konsentrasi 3,125%. Kesimpulan: Ekstrak kulit
nanas (Ananas comosus) efektif dalam menghambat maupun membunuh bakteri
Staphylococcus aureus. | en_US |
| dc.description.abstract | Background : Oral disease rank first by 60% in Indonesia. One of the bacteria that
causes oral disease is Staphylococcus aureus. Staphylococcus aureus is bacteria that
causes gingivitis and belong to gram positive, anaerobic. Pineapple (Ananas comosus)
is a widely spread plant which can live in highlands as well as in lowlands throughout
the globe. Active substances found in pineapple (Ananas comosus) peel have
antibacterial effect.
Objective : The purpose of this study was to determine the minimum inhibitory
concentration and minimum bactericidal concentration of pineapple (Ananas
comosus) peel extract with 100%, 50%, 25%, 12,5%, 6,25%, 3,125%, 1,56%, and
0,78% concentrations on Staphylococcus aureus bacteria (in vitro).
Method : This study was an in vitro, pure-laboratory experimental.. The subjects in
this study were Staphylococcus aureus and pineapple (Ananas comosus) peel extract.
Antibacterial effect test was conducted using liquid dilution method on Brain Heart
Infusion (BHI) medium and solid dilution method on Triton Soya Agar (TSA) medium
pineapple (Ananas comosus) peel extract was serially diluted into some
concentrations: 100%, 50%, 25%, 12.5%, 6.25%, 3.125%, 1.56%, and 0.78%.
Minimum inhibitory concentration and minimum bactericidal concentration were
determined by observing the growth of Staphylococcus aureus on both medium.
Result: Minimum Inhibitory Concentration (MIC) was at 1,56%, while Minimum
Bactericidal Concentration (MBC) was at 3,125%.
Conclusion: Pineapple (Ananas comosus) peel extract was effective in inhibiting and
eliminating Staphylococcus aureus. | en_US |
| dc.publisher | FAKULTAS KEDOKTERAN DAN ILMU KESEHATAN UNIVERSITAS MUHAMMADIYAH YOGYAKARTA | en_US |
| dc.subject | antibacterial effect, Staphylococcus aureus, pineapple peel, dilution method | en_US |
| dc.title | EFEKTIVITAS EKSTRAK KULIT NANAS (Ananas comosus) TERHADAP BAKTERI Staphylococcus aureus (Secara in vitro) | en_US |
| dc.type | Thesis
SKR
FKIK
206 | en_US |
