| dc.description.abstract | The purpose of this research wa^s to obtain the proper sterilization
method for ilg (Ficus caricaL.) shoot and determine the best combination of BAP
and NAA for in vitro shoot induction offig. The sterilization research was arranged
in completely randomized design (CRD), a single factor with five treatments. The
treatments tested were dipping in NaCIO (0.1, 57o for 1 min), fungicide and bactericide
4 g/L (5 min, 30 min, t h, 2 h, 3 h). Each treatment was repeated three
times. Parameters measured were the percentage of browning explants, the percentage
of contaminated explants and the percentage of survive explants. Shoots
induction research was arranged in completely randomized design (CRD), a single
factor with seven treatments. The treatments tested were the addition of BAP (0, 2.
4, 6 mgll) and NAA (0, 0.5, I mg/L) to the MS medium containing GA3. Each
treatment was repeated six times. Parameters measured were the percentage of
contaminated explants, the percentage of survive explants, percentage of shoot
formation, percentage of browning explants, number of shoots, shoot heighl,
number of leaves and percentage of rooted explants. The best method for fig shoot
sterilization was dipping explants in NaCIOlOTo, 5'+ NaCIO 5Vo,7' + fungicide
and bactericide 4 glL for 3 h with the percentage of survival explants 100% during
30 days of observation. The addition of 2 mgll- BAP and 0.5 mgil NAA into MS
containing GA3 medium was favorable to produce among the highest percentage of
fig shoot fbnnation (33.33Vo) and the browning explants (O.00Ec). | en_US |
