| dc.contributor.advisor | RINEKSANE, INNAKA AGENG | |
| dc.contributor.advisor | SUPANGKAT, GATOT | |
| dc.contributor.author | ETIKASARI, AMALIA PUJI | |
| dc.date.accessioned | 2020-02-26T03:08:28Z | |
| dc.date.available | 2020-02-26T03:08:28Z | |
| dc.date.issued | 2019-08-01 | |
| dc.identifier.uri | http://repository.umy.ac.id/handle/123456789/32036 | |
| dc.description | The research aims to determine the best type of medium and 2,4-D
concentration on somatic embryo induction of Vanda tricolor tissue culture. The
research was conducted in December 2018 to March 2019 at the Tissue Culture
Laboratory of Agriculture Faculty, Universitas Muhammadiyah Yogyakarta. The
research was conducted with a single factor experimental method and was
arranged in a Completely Randomized Design (CRD) with 3 replications. The
treatment tested was a combination of medium types (NDM and POC) with 2,4-D
concentrations (0; 1; 3; 5 mg/l). TDZ 0,5 mg/l was added to each treatment.
Parameters observed included the percentage of live explants, percentage of
browning explants, percentage of contaminated explants, percentage of vitrified
explants, time appears of pro-embryo, total of pro-embryos, percentage of callus
explants, time appears of callus, callus diameter, callus texture, explant height,
total of leaves, leaf color, and microscope observation. The results showed that
the use of POC medium with a concentration of 1 mg/l 2,4-D tended to give the
best effect as indicated by the time appears of pro-embryo at 2,72 weeks; total of
pro-embryos as much as 2,44 pro-embryos; the percentage of callus explants of
0,84%; time appears of callus at 30,50 days; and callus diameter of 3,13 mm. | en_US |
| dc.description.abstract | The research aims to determine the best type of medium and 2,4-D
concentration on somatic embryo induction of Vanda tricolor tissue culture. The
research was conducted in December 2018 to March 2019 at the Tissue Culture
Laboratory of Agriculture Faculty, Universitas Muhammadiyah Yogyakarta. The
research was conducted with a single factor experimental method and was
arranged in a Completely Randomized Design (CRD) with 3 replications. The
treatment tested was a combination of medium types (NDM and POC) with 2,4-D
concentrations (0; 1; 3; 5 mg/l). TDZ 0,5 mg/l was added to each treatment.
Parameters observed included the percentage of live explants, percentage of
browning explants, percentage of contaminated explants, percentage of vitrified
explants, time appears of pro-embryo, total of pro-embryos, percentage of callus
explants, time appears of callus, callus diameter, callus texture, explant height,
total of leaves, leaf color, and microscope observation. The results showed that
the use of POC medium with a concentration of 1 mg/l 2,4-D tended to give the
best effect as indicated by the time appears of pro-embryo at 2,72 weeks; total of
pro-embryos as much as 2,44 pro-embryos; the percentage of callus explants of
0,84%; time appears of callus at 30,50 days; and callus diameter of 3,13 mm. | en_US |
| dc.publisher | FAKULTAS PERTANIAN UNIVERSITAS MUHAMMADIYAH YOGYAKARTA | en_US |
| dc.subject | Vanda tricolor, New Dogashima Medium (NDM), Liquid Organic Fertilizer (POC), 2,4-Dichlorophenoxyacetic acid | en_US |
| dc.title | INDUKSI EMBRIO SOMATIK ANGGREK Vanda tricolor PADA MEDIUM NDM DAN PUPUK ORGANIK CAIR (POC) DENGAN PERLAKUAN 2,4-D | en_US |
| dc.type | Thesis SKR FP 262 | en_US |
