| dc.contributor.advisor | WIDADA, HARI | |
| dc.contributor.author | SHOLIKHAH, MAR’ATUS | |
| dc.date.accessioned | 2020-03-02T02:53:58Z | |
| dc.date.available | 2020-03-02T02:53:58Z | |
| dc.date.issued | 2019-07-22 | |
| dc.identifier.uri | http://repository.umy.ac.id/handle/123456789/32177 | |
| dc.description | Gold nanoparticles are platforms that can be immobilized with probe, so
they can be used as biosensors. In health sector, biosensors are used for biomedical
applications, such as for DNA marking and cell isolation. In addition, it can be used
as a method for Halal Authenticaton applications for meat products. This study
aims to determine the optimum conditions for the synthesis of gold nanoparticles as
platform for immobilization and application of AuNPs-MB as biosensors with the
colorimetry method for Halal Authentication applications for meat product.
This research was optimize synthesis of gold nanoparticles using 6 series
of HAuCl4 concentration, there are 0.0635; 0.127; 0.19; 0.254; 0.635; dan 1.27
(mM). The most optimum concentration, then immobilized with probe MB and
measured using Uv-Vis Spectrophotometer to see the absorption and using
Scanning Electron Microscope (SEM) to see the size and distribution of
nanoparticles.
The results showed that 0.19 mM of AuNPs, gave the best results, which
was stable for 3.5 months, has good results by measured using SEM with an
average size of 21.7 nm. The value absorbance before to after immobilization was
decreased, from 0.226 to 0.167 and indicate that the proses is success. The
conclusion of this study is AuNPs-MB is proven to be a biosensor for Halal
Authentication applications using colorimetric method. | en_US |
| dc.description.abstract | Gold nanoparticles are platforms that can be immobilized with probe, so
they can be used as biosensors. In health sector, biosensors are used for biomedical
applications, such as for DNA marking and cell isolation. In addition, it can be used
as a method for Halal Authenticaton applications for meat products. This study
aims to determine the optimum conditions for the synthesis of gold nanoparticles as
platform for immobilization and application of AuNPs-MB as biosensors with the
colorimetry method for Halal Authentication applications for meat product.
This research was optimize synthesis of gold nanoparticles using 6 series
of HAuCl4 concentration, there are 0.0635; 0.127; 0.19; 0.254; 0.635; dan 1.27
(mM). The most optimum concentration, then immobilized with probe MB and
measured using Uv-Vis Spectrophotometer to see the absorption and using
Scanning Electron Microscope (SEM) to see the size and distribution of
nanoparticles.
The results showed that 0.19 mM of AuNPs, gave the best results, which
was stable for 3.5 months, has good results by measured using SEM with an
average size of 21.7 nm. The value absorbance before to after immobilization was
decreased, from 0.226 to 0.167 and indicate that the proses is success. The
conclusion of this study is AuNPs-MB is proven to be a biosensor for Halal
Authentication applications using colorimetric method. | en_US |
| dc.publisher | FAKULTAS KEDOKTERAN DAN ILMU KESEHATAN UNIVERSITAS MUHAMMADIYAH YOGYAKARTA | en_US |
| dc.subject | Gold Nanoparticles, Molecular Beacon, Biosensor, Immobilization, Colorimetry | en_US |
| dc.title | OPTIMASI NANOPARTIKEL EMAS (AuNP) SEBAGAI PLATFORM IMOBILISASI PROBE MOLECULAR BEACON (MB) UNTUK APLIKASI AUTENTIKASI HALAL DENGAN METODE KOLORIMETRI | en_US |
| dc.type | Thesis SKR FKIK 435 | en_US |
