| dc.contributor.author | HIDAYATI, TITIEK | |
| dc.contributor.author | PRAMONO, ARDI | |
| dc.contributor.author | JENIE, IKHLAS MUHAMMAD | |
| dc.contributor.author | SOESATYO, MARSETYAWAN HNE | |
| dc.date.accessioned | 2016-09-30T15:02:35Z | |
| dc.date.available | 2016-09-30T15:02:35Z | |
| dc.date.issued | 2016 | |
| dc.identifier.citation | www.biomedres.info | en_US |
| dc.identifier.issn | 0970-938X | |
| dc.identifier.uri | http://repository.umy.ac.id/handle/123456789/4238 | |
| dc.description.abstract | Dimethylbenzanthracene (DMBA) compounds were proven to suppress bone marrow activity,
immunosuppressive, and genotoxic and carcinogenic. Black cumin seed extract was used as an
immunomodulator, which can be developed as a great therapeutic agent for adjuvant chemotherapy in
cancer patients as an immunomodulatory agent. The aim of this study is to determine the effect of black
cumin seed hexane extract (BCSHE) as an immunomodulator in SD rat’s peritoneal macrophages
induced by DMBA or without DMBA. In vitro experimental study was performed on rat’s peritoneal
macrophages cultures induced by DMBA and without DMBA. BCSHE were administered to cultures in
five different doses; 1, 5, 25, 125 and 625 μg/ml with control media, tween, and positive control
thymoquinone. The test substance was added after the macrophages were activated and then measured
by using latex phagocytosis method while ROI secretion assay was measured by using NBT assay
method. Results showed that macrophage activation by BCSHE solution increased ROI secretory activity in both SD rat’s macrophage induced by DMBA and without DMBA. BCSHE could increase phagocytic activity in-vitro. It can be concluded that BCSHE can increase ROI secretion and phagocytic activity on SD rat’s peritoneal macrophages induced by DMBA and without DMBA | en_US |
| dc.language.iso | en_US | en_US |
| dc.publisher | Biomedical Research | en_US |
| dc.relation.ispartofseries | 28 (3); | |
| dc.source | Biomed Res- India 2017 Volume 28 Issue 3 | |
| dc.source.uri | www.biomedres.info | |
| dc.subject | Black cumin seeds hexane extract | en_US |
| dc.subject | Dimethylbenzanthracene | |
| dc.subject | Immunomodulator | |
| dc.subject | Macrophages | |
| dc.title | EVALUATION OF BLACK CUMIN SEEDS HEXANE EXTRACT AS REACTIVE OXYGEN INTERMEDIATES (ROI) AND PHAGOCYTIC ACTIVITY MODULATOR IN DMBA INDUCEDRATS. | en_US |
| dc.type | Article | en_US |
