| dc.contributor.advisor | ANDRIANI, IKA | |
| dc.contributor.author | PUTRI, RIZKI DWIDYANI | |
| dc.date.accessioned | 2016-11-17T06:41:50Z | |
| dc.date.available | 2016-11-17T06:41:50Z | |
| dc.date.issued | 2016-03-07 | |
| dc.identifier.uri | http://repository.umy.ac.id/handle/123456789/6441 | |
| dc.description | Penyakit periodontal menduduki urutan kedua masalah gigi di masyarakat. Bakteri Aggregatibacter actinomycetemcomitans merupakan penyebab periodontitis yang termasuk dalam golongan bakteri gram negatif, anaerob. Nanas (Ananas comosus) adalah tanaman yang telah tersebar luas ke seluruh dunia dan dapat tumbuh di dataran rendah maupun dataran tinggi. Pada bagian kulit buah nanas (Ananas comosus) mengandung Flavonoid dan enzim bromelin yang mampu menghambat pertumbuhan bakteri.
Tujuan: penelitian ini bertujuan untuk mengetahui Kadar Hambat Minimal (KHM) dan Kadar Bunuh Minimal (KBM) dari ektrak kulit nanas (Ananas comosus) terhadap pertumbuhan Aggregatibacter actinomycetemcomitans.
Metode: Jenis penelitian adalah penelitian ekperimental semu laboratoris (in vitro). Uji daya antibakteri dilakukan dengan metode dilusi cair pada media Brain Heart Infusion (BHI) dan metode dilusi cair pada media Triton Soya Agar (TSA). Ekstrak kulit nanas (Ananas comosus) diencerkan dengan cara berseri kedalam beberapa konsentrasi: 100%, 50%, 25%, 12,5%, 6,25%, 3,125%, 1,56%, 0,78%, dan 0,39%. Kadar hambat minimal dan kadar bunuh minimal ditentukan dengan mengamati dari pertumbuhan bakteri Aggregatibacter actinomycetemcomitans media BHI dan TSA.
Hasil: Kadar Hambat Minimal (KHM) dan Kadar Bunuh minimal (KBM) terdapat pada konsentrasi 6,25%.
Kesimpulan: Ekstrak kulit nanas (Ananas comosus) efektif dalam menghambat maupun membunuh bakteri Aggregatibacter actinomycetemcomitans | en_US |
| dc.description.abstract | Periodontitis comes as the second most common dental disease problem in society. Aggregatibacter actinomycetemcomitans bacteria belongs to anaerobic gram-negative group known as the main cause of agrresive periodonitis. Pineapple (Ananas comosus) is a widely spread plant which can live in highlands as well as in lowlands throughout the globe. Flavonoid and bromelain enzyme found in pineapple (Ananas comosus) skin can be used as a bacterial growth inhibitor.
Objective: The purpose of this study was to determine the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of pineapple (Ananas comosus) skin extract on the growth of Aggregatibacter actinomycetemcomitans.
Method: This study was an in vitro, semi-laboratory experiment Antibacterial effect test was conducted using liquid dilution method on Brain Heart Infusion (BHI) medium and solid dilution method on Triton Soya Agar (TSA) medium Pineapple (Ananas comosus) skin extract was serially diluted into some concentrations: 100%, 50%, 25%, 12.5%, 6.25%, 3.125%, 1.56%, 0.78%, and 0.39%. Minimum inhibitory concentration and minimum bactericidal concentration were determined by observing the growth of Aggregatibacter actinomycetemcomitans on both medium.
Result: Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) was found at 6,25%.
Conclusion: Pineapple (Ananas comosus) skin extract was effective in inhibiting and eliminating Aggregatibacter actinomycetemcomitans | en_US |
| dc.publisher | FKIK UMY | en_US |
| dc.subject | antibacterial effect, Aggregatibacter actinomycetemcomitans, pineapple peel, dilution method.. daya antibakteri, Aggregatibacter actinomycetemcomitans, kulit nanas, metode dilusi. | en_US |
| dc.title | DAYA ANTIBAKTERI EKSTRAK KULIT BUAH NANAS (Ananas comosus) TERHADAP PERTUMBUHAN BAKTERI Aggregatibacter actinomycetemcomitans | en_US |
| dc.type | Thesis
SKR
333 | en_US |
