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dc.contributor.authorASTUTI, AGUNG
dc.contributor.authorYUWONO, TRIWIBOWO
dc.contributor.authorMOELJOPAWIRO, SUKARTI
dc.date.accessioned2016-12-13T05:18:22Z
dc.date.available2016-12-13T05:18:22Z
dc.date.issued2001-02
dc.identifier.urihttp://repository.umy.ac.id/handle/123456789/7149
dc.description.abstractActinidin is an intracellular cysteine protease found in the fruit of Chinese gooseberry (Actinidia chinensis) or Kiwi fruit. A variant of actinidin-encoding DNA sequence has been constructed by amplifying a specific region of the full-length actinidin cDNA using PCR method. Amplification was targeted to construct an actinidin-encoding DNA sequence consisting of mature actinidin-encoding DNA plus part of the N-terminal extension (carrying the QRTN motif) and part of the C-terminal extension without QR sequence motif. The amplified fragment was then cloned in an expression-secretion vector (pYSV9) and expressed in Saccharomyces cerevisiae. The results of this study demonstrated that the cloned fragment had a smaller size as compared to the PCR amplification product, suggesting that a rearrangement event occured during the cloning process. Yeast culture supernatant was analysed for expression of actinidin by using anti-actinidin polyclonal antibody raised in chicken. The expression of the cloned fragment in S. cerevisiae, however, did not result in any detectable expression product on the immunoblot.en_US
dc.subjectActinidinen_US
dc.subjectexpression and secretion
dc.subjectSaccharomyces cerevisiae
dc.titleTHE ENGINEERING OF ACTINIDIN PROTEIN AND ITS EXPRESSION IN THEYEAST SACCHAROMYCES CEREVISIAEen_US
dc.typeArticleen_US


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