| dc.contributor.advisor | TASMINATUN, SRI | |
| dc.contributor.author | RAHMAWATI, IKA DEWI | |
| dc.date.accessioned | 2016-12-19T03:10:58Z | |
| dc.date.available | 2016-12-19T03:10:58Z | |
| dc.date.issued | 2016-08-22 | |
| dc.identifier.uri | http://repository.umy.ac.id/handle/123456789/7312 | |
| dc.description | Pumpkin (Cucurbita moschata Duch Poir) is one of the plants that are easily found in Indonesia, but has not been used optimally. C. moschata seeds are known to contain many different active compounds such as flavonoid and alkaloid. Flavonoid and alkaloid known to give the effect of antibacterial and anti radical. Free radicals is one of the main causes of the occurrence of degenerative diseases. The infection caused by bacteria may also aggravate a degenerative disease complications. This research aims to identify and investigate the effect of flavonoid and alkaloid compounds of ethanolic extract seeds of C. moschata as antioxidant and antibacteria.
The seeds of C. moschata were extracted using maceration method with ethanol 70%. The extract of C. moschata seeds was identified flavonoid and alkaloid compounds using thin layer chromatography methods. Then tested its antioxidant activity using DPPH method and the antibacteria was tested by disc diffusion method. The data % inhibition got on the antioxidant activity testing poured into a curve between the % inhibition and the concentration to get the equivalence of linear regression. The equivalence of linear regression was used to decide Inhibitory Concentration (IC50). On antibacteria activity testing, it was done with diameter zone of inhibition measurement method toward Staphylococcus aureus bacteria, and then compared with the standard value of antibacterial activity.
The results of the identification showed the extract ethanolic of C. moschata seeds contain of flavonoid and alkaloid compounds with Rf 0.87 and 0.56. Compared to standard quercetin and kuinin compounds that showed Rf 0.78 and 0.56. This study showed that extracts ethanolic of C. moschata seeds had weak antioxidant activity with IC50 values of 420.08 μg/ml and has antibacterial effects on concentration of 25% (1.31 mm) and 50% (2.24 mm) toward the S. aureus bacteria. | en_US |
| dc.description.abstract | Pumpkin (Cucurbita moschata Duch Poir) is one of the plants that are easily found in Indonesia, but has not been used optimally. C. moschata seeds are known to contain many different active compounds such as flavonoid and alkaloid. Flavonoid and alkaloid known to give the effect of antibacterial and anti radical. Free radicals is one of the main causes of the occurrence of degenerative diseases. The infection caused by bacteria may also aggravate a degenerative disease complications. This research aims to identify and investigate the effect of flavonoid and alkaloid compounds of ethanolic extract seeds of C. moschata as antioxidant and antibacteria.
The seeds of C. moschata were extracted using maceration method with ethanol 70%. The extract of C. moschata seeds was identified flavonoid and alkaloid compounds using thin layer chromatography methods. Then tested its antioxidant activity using DPPH method and the antibacteria was tested by disc diffusion method. The data % inhibition got on the antioxidant activity testing poured into a curve between the % inhibition and the concentration to get the equivalence of linear regression. The equivalence of linear regression was used to decide Inhibitory Concentration (IC50). On antibacteria activity testing, it was done with diameter zone of inhibition measurement method toward Staphylococcus aureus bacteria, and then compared with the standard value of antibacterial activity.
The results of the identification showed the extract ethanolic of C. moschata seeds contain of flavonoid and alkaloid compounds with Rf 0.87 and 0.56. Compared to standard quercetin and kuinin compounds that showed Rf 0.78 and 0.56. This study showed that extracts ethanolic of C. moschata seeds had weak antioxidant activity with IC50 values of 420.08 μg/ml and has antibacterial effects on concentration of 25% (1.31 mm) and 50% (2.24 mm) toward the S. aureus bacteria. | en_US |
| dc.publisher | FKIK UMY | en_US |
| dc.subject | antibacteria, antioxidant, Cucurbita moschata, Staphylococcus aureus. | en_US |
| dc.title | UJI AKTIVITAS ANTIOKSIDAN DAN ANTIBAKTERI EKSTRAK ETANOLIK BIJI LABU KUNING (Cucurbita moschata Duch Poir) | en_US |
| dc.type | Thesis
SKR
371 | en_US |
