Hospital as health care organization must develop a patient safety culture in an effort to improve the quality of care in order to prevent the accurrence of adverse event. MaPSaF (Manchester Patient Safety Framework) is one of instrument that published by National Patient Safety Agency (NPSA) in 2006, it can be used to measure the level of patient safety culture. The aim of this study was to determine the dimensions of the patient safety culture instrument by MaPSaF. This study was a quantitative design with study literature approach. The results of this study was MaPSaF composed of 10 dimensions of patient safety culture with 24 themes. The dimensions are commitment to overall continous improvement, priority given to safety, system errors and individual responsibility, recording incidents and best practice, evaluating incidents and best practice, learning and affecting change, communication about safety issues, personnel management and safety issues, staff education and training, and team working. The themes are commitment to improvement, audit,policies, priority of patient safety, risk management system, implementation of patient safety, the cause of the incident, patient safety culture, reporting feeling and system, data analysis, the focus and result of investigation, incidents learning, the people in deciding of change, communication about patient safety between staf, patient or both, share the information, supporting the staff, training needs andpurposes, team structure, the  ow of information and sharing. The conclusion of this study was MaPSaF tools suitable for assessing the level of patient safety culture at the hospital in Indonesia.