| dc.contributor.author | MAKIYAH, SRI NABAWIYATI NURUL | |
| dc.contributor.author | TASMINATUN, SRI | |
| dc.contributor.author | RIFA’I, MUHAIMIN | |
| dc.contributor.author | WIDODO, WIDODO | |
| dc.contributor.author | DJATI, MUHAMMAD SASMITO | |
| dc.date.accessioned | 2017-09-07T03:45:04Z | |
| dc.date.available | 2017-09-07T03:45:04Z | |
| dc.date.issued | 2016-12-09 | |
| dc.identifier.isbn | 978-602-757-793-0 | |
| dc.identifier.uri | http://repository.umy.ac.id/handle/123456789/14362 | |
| dc.description | Purple yam (Dioscorea alata L.) is a source of biological tubers not been used
optimally. Diosgenin is steroids saponin compounds that most important because it has
multiple biological functions, such as allergenic activity. The objective of this research
was to analyze the potential allergenic ethanol extract tuber of D. alata L. (EEDA) on
BALB/c mice on sensitivity phase with measure the pro le of transcription factor FoxP3
Treg cells and cytokine pro le of Treg and IgE and IgG1 B cells. An experimental study
using BALB/c mice divided into 7 groups: control group (I), the treatment group (II-V)
ethanol extract of tuber Dioscorea alata L. dose of 0.00; 0.17; 2.01; 10.04 g/kg bw, the
treatment group antihistamines drug and Diosgenin (VI-VII). For 17 consecutive days
the group II-VII were treated in accordance with the group and with Ovalbumin induced
allergy models. Mice were sacri ced on day 18. Spleen is removed, lymphocyte isolated
and analyze the transcription factor FoxP3 Treg cells, cytokine pro le of T reg cells
and IgE and IgG1 B cells on spleen using Flowcytometry FACS Calibur. The results
showed EEDA able to inhibit the production of B220IgE and B220IgG1, trigger Treg
cells (CD4CD25) and the transcription factor FoxP3 (CD4CD25FoxP3) and pro le of the
cytokines produced by T reg cells. The conclusion is ethanol extract tubers of Dioscorea
alata L (EEDA) does not trigger Treg (CD4CD25) and the transcription factor FoxP3
(CD4CD25FoxP3) and cytokine pro les produced by Treg cells namely CD4IL-10,
CD8IL-10, CD4TGF-β and CD8TGF -β in the sensitivity phase. | en_US |
| dc.description.abstract | Purple yam (Dioscorea alata L.) is a source of biological tubers not been used
optimally. Diosgenin is steroids saponin compounds that most important because it has
multiple biological functions, such as allergenic activity. The objective of this research
was to analyze the potential allergenic ethanol extract tuber of D. alata L. (EEDA) on
BALB/c mice on sensitivity phase with measure the pro le of transcription factor FoxP3
Treg cells and cytokine pro le of Treg and IgE and IgG1 B cells. An experimental study
using BALB/c mice divided into 7 groups: control group (I), the treatment group (II-V)
ethanol extract of tuber Dioscorea alata L. dose of 0.00; 0.17; 2.01; 10.04 g/kg bw, the
treatment group antihistamines drug and Diosgenin (VI-VII). For 17 consecutive days
the group II-VII were treated in accordance with the group and with Ovalbumin induced
allergy models. Mice were sacri ced on day 18. Spleen is removed, lymphocyte isolated
and analyze the transcription factor FoxP3 Treg cells, cytokine pro le of T reg cells
and IgE and IgG1 B cells on spleen using Flowcytometry FACS Calibur. The results
showed EEDA able to inhibit the production of B220IgE and B220IgG1, trigger Treg
cells (CD4CD25) and the transcription factor FoxP3 (CD4CD25FoxP3) and pro le of the
cytokines produced by T reg cells. The conclusion is ethanol extract tubers of Dioscorea
alata L (EEDA) does not trigger Treg (CD4CD25) and the transcription factor FoxP3
(CD4CD25FoxP3) and cytokine pro les produced by Treg cells namely CD4IL-10,
CD8IL-10, CD4TGF-β and CD8TGF -β in the sensitivity phase. | en_US |
| dc.language.iso | en | en_US |
| dc.publisher | Department of Histology & Biology, Medical Science Study Programe Faculty of Medicine & Health Science, Universitas Muhammadiyah Yogyakarta. Pharmacy Study Programme, Faculty of Medicine & Health Science, Universitas Muhammadiyah Yogyakarta. Biology Department, Faculty of Mathematic and Sciences, Brawijaya University | en_US |
| dc.subject | Saponin steroid of ethanol extract of the tubers of Dioscorea alata L.; allergenic agent; Treg cells; the transcription factor FoxP3; lymphocyte. | en_US |
| dc.title | STEROIDAL SAPONIN IN ETHANOL EXTRACT TUBER OF PURPLE YAM (Dioscorea alata L. ) AS ALLERGENIC AGENT ON SENSITIVITY PHASE BALB/C MICE MODEL ALLERGY ON TRANSCRIPTION FACTOR FOXP3 AND CYTOKINE PROFILES OF T REG | en_US |
| dc.type | Book | en_US |
