| dc.contributor.author | FAUZI, AKHMAD FARIED | |
| dc.date.accessioned | 2017-10-17T03:13:09Z | |
| dc.date.available | 2017-10-17T03:13:09Z | |
| dc.date.issued | 2017-08-18 | |
| dc.identifier.uri | http://repository.umy.ac.id/handle/123456789/15526 | |
| dc.description.abstract | Background : Propolis is a dark and sticky substance produced by bees. Propolis in the world of health was known to have benefits as anti-inflammatory, antioxidant, antibacterial, antiviral, antifungal, and antitumor. However, propolis has the potential of being toxic if used as herbal medicine. Purpose : This study aim is to tests the effect of cytotoxic ethanol extract of propolis (EEP) on viability of cultured fibroblast cells.. Method : The design of this study was pure laboratory experimental in vitro. Propolis used comes from Apis trigona bees and extracted using 40% ethanol. EEP is then used as a treatment on Human Dermal Fibroblast adult (HDFa) cells. The cytotoxic effects of EEP were analyzed based on viability of fibroblast cells calculated by the method of MTT assay. Results : EEP with a concentration of 500 μg / mL had the highest cytotoxic effect because it resulted in a culture of fibroblasts having viability of 8.44%. EEP-treated fibroblast cells show a morphological change observed under a microscope. Morphological changes show few cells become smaller and rounder. Statistical analysis was done by using Spearman correlation test. The coefficient of correlation shows (r = 0,839) which means there is a strong and opposite relationship between giving EEP various concentrations and viability of fibroblast cells. Conclusion : EEP showed the presence of dose-dependent cytotoxicity on fibroblasts' viability. | en_US |
| dc.publisher | FAKULTAS KEDOKTERAN DAN ILMU KESEHATAN UNIVERSITAS MUHAMMADIYAH YOGYAKARTA | en_US |
| dc.subject | Ethanol Extract of Propolis | en_US |
| dc.subject | Human Dermal Fibroblast adult | en_US |
| dc.subject | Cell Viability | en_US |
| dc.subject | MTT Assay | en_US |
| dc.title | UJI SITOTOKSISITAS EKSTRAK ETANOL PROPOLIS LEBAH Apis trigona TERHADAP VIABILITAS KULTUR FIBROBLAS | en_US |
| dc.type | Thesis | en_US |
