With an assortment of new motorcycle can not eliminate the desire of consumers to keep caring for two stroke motorcycle, as known by a motorcycle that has an engine construction is simple and a great power. Lubrication system used on the two stroke motorcycle different from the four stroke motorcycle, lubrication system on the motor two steps equipped with a two stroke oil that will be lubricate the components inside the cylinder and then join the burn in gasoline, This will also affect the performance of the machine. This research aims to know the influence of the use of 3 types of two stroke oil on performance Motorcycle Kawasaki Ninja RR 150 cc 2014. Testing is done using 3 samples oil two stroke, oil Shell Advance SX 2T, Mesrania 2T Super, and Ultralin Racing 2T from Premium fuel, using Viscosity test, Dynometer testing and fuel consumption. After doing the test the viscosity can be drawn the conclusion that oil Ultralin has a high viscosity value while lowest viscosity is present on the oil Mesrania but at the moment the temperature ± 60 ° C the value of the oil is not too much difference in its viscosity. While the value of torque and power is present on the Shell Oil 2T has the greatest torque value i.e. 18.82 N.m on lap machine 8987 rpm And the value of power i.e. 24.4 HP at 9500 rpm engine rotation, on testing the fuel consumption the two stroke oil of the least fuel consuming namely oli ultralin racing 2T with 16.89 Km/l value. with an average speed of 34.44 km/h.