Polyvinyl alcohol (PVA) is one of the conductive polymers which potentially uses for wound dressing material due to its beneficial properties such as biodegradable, biocompatible and non-toxic. Nanofiber membranes of PVA were prepared by electrospinning method to characterize the fiber morphology and tensile properties of PVA nanofiber membranes, and the relationship between them. In this work, PVA GOHSENOL with various concentrations of (6%, 8% and 10% w/w) and PVA (Mw: 85.000 – 124.000) with a concentration of 10% w/w were used as polymer solutions in the electrospinning process. Preparation of PVA nanofiber membranes was carried out at constant parameters of high DC voltage 25 kV, spinneret diameter 0.7 mm and a distance between the tip and metallic collector (TCD) 12 cm. The fiber morphology of PVA membranes was characterized by scanning electron microscopy (SEM, Hitachi TM3030). A universal testing machine (UTM, Zwick 0.5 Germany) was used for tensile test of the nanofiber membranes. The tensile test specimen was prepared according to ASTM D 882. The results showed that the average fiber diameter increases with increasing concentration of polymer solution made of PVA GOHSENOL and fiber diameter of a polymer solution made of PVA (Mw: 85.000 – 124.000) is higher than that of PVA GOHSENOL. Those fiber diameters are ranging from 356 nm to 551 nm. Enhancement of fiber diameter seems to possess a correlation effect on the increase of tensile properties of the PVA nanofiber membranes. Tensile strength (3.27 – 15.42 MPa), tensile modulus (17.53 – 83.07 MPa) and elongation (23.01 – 89.95%) of PVA nanofiber membranes are included in the range of a standard material for the biomedical application.