Neonatal sepsis is a major cause of morbidity and mortality in newborn. As the clinical manifestations are vague and non-specific, it is pertinent that an early diagnosis is made to prevent serious morbidity and mortality. Neonatal sepsis is a clinical syndrome resulting from the pathophysiological effects of very severe bacterial infections that occur in the first month of life. Infection that occurs in the neonate can cause some changes in blood cells either erythrocytes, leukocytes, or platelet. The purpose of this study was to determine the relationship between I/T ratio and neutrophil count in neonatal sepsisto accelerate the diagnostic process. The research methode used is analytic observational prospective study with cross sectional design, Research subjects are 103 neonates with sepsis which recorded on medical record from January 2014 to March 2017 at RS PKU Muhammadiyah Yogyakarta. Analysis of data used spearman correlation test to determine the relationship of I / T Ratio with neutrofil count in neonatal sepsis. The results of the secondary data on 103 patients who had sepsis risk gained prevalence of neonatal sepsis based on onset, early onset sepsis (<72 hours) is higher than the late onset sepsis (66.9%; 33.1%) by sex obtained group sex male is higher than the female gender group (58.3%; 41.7%). The average of the result of I / T ratio 0.16, an average birth weight 2731, and neutrophil average 52,65 %. Spearman correlation analysis showed a significant correlation between I / T ratio with neutrophil count p =0,127.