Background: Mosquito Repellent is a drug that used to eradicate the mosquitoes in a room. nowadays, there are many variation of a mosquito repellent that distribute in the market.as example is a spray mosquito repellent that contain a toxic subtances like pralethrin (0,1%), sifultrin (0,05%) and d-Allethrin (0,57%). In the one push mosquito repellent contain a toxic subtance called transflutrin (21,3%). The goal of this study is to analyze the effect of spray mosquito repellent and one push mosquito repellent against the diameter of tubulus seminiferus and the amount of sperm in rat (rattus norvegicus). Methods: the design of this study is laboratoric experimental with post-test only control group design. This study use 30 rat (Rattus novergicus) with the age of 2 months and divided into 5 group. The data is taken, and then the diamater of tubulus seminiferus is measured and the amount of sperm is calculated. Result: the result of one-way ANOVA statictic show that there is meaningful difference in the amount of sperm (p<0,05) with the result of the 10 minutes spray group (P4) that have lowest amount of sperm. The Kruskal-Wallis result show that there is meaningful differences in diameter of tubulus seminiferus (p<0,05) with the result of 10 minutes spray group (P4) that have smallest diameter. Conclusion: the differenciation of spray mosquito repellent and one push mosquito repellent can affect the diameter of tubulus seminiferus and the amount of sperm.