Background : The use of mosquitos repellent has been frequently in demand by society. This is proven by its new innovation developed in the new kind of mosquito spray repellent is one push. There are difference between conventional spray repellent in terms of their active substances. Spray repellent contains sifultrin, praletrin, and d-alletrin, meanwhile, in one push repellent it contains transfultrin. These active substance are not yet warranted to be safe for human respiration system, so that a thorough experiment concerning the effects of using spray repellent and one push repellent to changes in pulmonary histology of Rattus norvegicus is needed. Method : using true eperiment by doing post test only control group design method. Subject experiment are 30 Wisttar rats (Rattus norvegicus) aged 2 months old divided into 6 on each control group (K), 5 minutes one push (P1), 10 minutes one push (P2), 5 minutes spray (P3), and 10 minutes spray (P4). Treatments are done 60 days followed with surgical operation to take samples and running a histopatological pulmo test. Result are then analysed using One Way Anova statistic test followed by post hoc Tukey test. Result : thickness of septum interalveolaris and alveolus diameter showed significant (P<0,005) between control group (K) with group (P1), (P2), (P3), and (P4). Conclusion : mosquito conventional spray repellent and one push repellent give negative effects to pulmonary histology representation.