Cockroach role as vectors of many diseases, as well as allergens. Eradication using chemical insecticide with risky accumulate on foods tuffs and through inhalation and damage the environment. Annona squamosa is potentially leaves as botanical insecticide as it is more environmentally friendly. This research aims to unravel the efficacy of Annona squamosa leaves extract to kill cockroach (Periplaneta americana). This research is experimentally with post-test only control group design. The research consists of seven groups treatment based on the concentration of the leaf extract (100%, 75%, 50%, 25%, 10%, 5%, and 0.1%), a positive control (deltamethrin 0.6%) and negative control (aquadest). Each group consists of five cockroaches with two replication for each treatment. Mortality number (%) after treatment were calculate and analyzed by one way anova. There are significant differences between the research groups (F calculated 41,25 > F table 3.32). Leaves extract 25%, 50%, 75% and 100% proved effective kill Periplaneta americana, but less effective when compared to the positive control (deltamethrin). Leaves extract 0.1%, 5% and 10% proved ineffective kill Periplaneta americana. The lethal concentration of leaves extract Annona squamosa (LD50) were 61,302% against cockroach.