62 Deteksi Resistensi Insektisida Nyamuk Aedes Aegypti Berdasarkan Aktifitas Enzim Glutation S-Transferase Detection of Aedes Aegypti Mosquito Resistance Based on Glutation-S-Tranaferase Enzyme Activity Sri Sundari 1 , Salmah Orbayinah 2 1 Bagian Parasitologi Program Studi Pendidikan Dokter Fakultas Kedokteran dan Ilmu Kesehatan Universitas Muhammadiyah Yogyakarta, 2 Bagian Biokimia Program Studi Pendidikan Dokter Fakultas Kedokteran Universitas Muhammadiyah Yogyakarta Email: sundari_purbo@yahoo.com.sg Abstract Organophosphate insecticide especially temephos and malathion have been used to eliminate mosquito and larvae Aedes aegypti in Yogyakarta province since 1974 up to now. Using by unappropriate chemical insecticide in long term causes resistance of mosquito to the insecticide. It indicates the increasing of Glutation-S-transferase and hydrolyses activity.The biochemical research method conducted at 1995 in Yogyakarta demonstrsted that mosquito and larvae Ae.aegypti based on enzyme esterase activity tend to be resist to organophosphate insecticide. The purpose of this research is to evaluate the resistance status of Ae. Aegypti mosquito to malathion insecticide in some villages in Bantul district based on Glutation-S- transferase activity. This research design was cross sectional study . Sample was collected randomly from these villages : Badegan, Palbapang and Bogoran in Bantul District. There were 48 mosquitos taken for examination of Glutation-S-tranferase activity.The result of examination based on Glutation-S-tranferase activity showed that the average of Ae. Aegypti mosquito resistance in three villages of Bantul District such as: moderate resistance (RS) 15%, susceptible (SS) 85%. However, statitiscally, there was no significant difference of resistance status of mosquito among three villages based on Glutation-S-tranferase (p>0,05)