Diabetes mellitus (DM) is ricing nowadays. DM is a metabolic deasease characterized by increasing of glucos e blood level for prolonged time. Patient with DM must take medicine for lo wering glucose levels in whole life. Synthetic anti-diabetic agent is comm only used and significantly able to maintain the glucose blood levels. However, many side effects will occur during the treatment. Further, peopl e are trying to find an alternative medicine for DM treatment. This pape r will discuss about an alternative medicine for anti-diabetic agent that is called Andrographis paniculata (Burm. f.) Nees based in-vivo study. Search data obtained from PubMed, PMC (PubMed Central), International Food and Research Journal, and Google search engine. Inclution and exclut ion criteria were used for selection of journals. Inclution cr iteria were taken is in-vivo study of Andrographis paniculata as anti-diabetic, published in 2000-2014, and free full text journal or PubReader (full text journal in PubM ed Central) in English or Indonesian language. Base on reviewed journals th at was conducted, anti-diabetic agent of Andrographis paniculata was found in extract, in fractional coumpund (i.e. andrographolide or its analogue AL-1: andrographolide-lipoic acid), and in combination with synthetic drug or other herbal. Therefore, Andrographis paniculata is potentially developed as an alternative anti-diabetic agent