Self regulated learning is a constuctive procces where a student determines their own learning goals, motivate and manage their behaviors to achieve a good result in learning. Learning planning will be more directed by goal orientation that becomes the reason and motivation of a person to achieve their learning goals. This research is conducted to discover the difference of self regulated learning from goal orientation in medical student of 2016 of Muhammadiyah Yogyakarta University. Method : The method employed for this study is quantitative comparison. The subject of research involves 60 students that are divided into two groups which are students with mastery goal and students with performance goal. The sampling technique is using probability sampling as simple random sampling that takes sample from a random population without considering the strata in the population. Research data is taken using self regulated learning scale and goal orientation scale. Self regulated learning scale consists of 39 valid items with an alpha coefficient of reliability 0,936. Goal orientation scale consists of 7 valid items for a mastery goal with an alpha coefficient of reliability 0,643 and 7 valid items for a performance goal with an alpha coefficient of reliability 0,711. Result : Based on difference test using t test terchnique (independent sample test) in SPSS 16.0 for windows, the value obtained is t = 4,740 with cognitive value or p = 0,000. The value means there is a difference of self regulated learning between students in terms of goal orientation. Based on the results of the analysis test, it is found that the students with mastery goal is better than the students with performance goal, this is seen from the empirical mean of students with mastery goal is higher than the empirical mean of students with performance goal (112,70>102,20). Conclusion : There is a significant difference between self regulated learning in terms of goal orientation of medical student of 2016 in Muhammadiyah Yogyakarta University.