This study aims to determine the effectiveness of the addition of nutrients molasses and liquid waste of tofu in the growth and production of oyster mushroom and determine the best concentrations of molasses and liquid waste of tofu for the growth of oyster mushroom. This research is compiled in CRD (completely randomized design) with the experimental design single factor that is baglog sawdust combined with molasses and nutrient enrichment liquid waste of tofu so there were four treatments in this study such as the addition of molasses 1 ml + liquid waste of tofu 40 ml, molasses 15 ml + 30 ml of liquid waste of tofu, molasses 20 ml + liquid waste of tofu 40 ml and 20 ml molasses + liquid waste of tofu 30 ml. The parameters of this study is observed the total time mycelial growth of 100%, the percentage of mycelial growth every week, baglog weight, the percentage of contamination, time of the first harvest, the amount of fruit weight, fresh weight and total weight of the harvested crop. The result of the research shows additional treatment of molasses 20 ml + 40 ml of liquid waste of tofu give the best results to the total time mycelial growth of 100% over the 28,50 days, while the percentage of growth of mycelium close 100% at week four, the time of harvest first for 33,08 days, the number of total fruit weight and total weight 64,66 fresh fruit harvest oyster mushroom as much as 432,14 grams.