Background: Indonesia has the second largest number of TB cases in the world. TB-DM is a new emerging double burden disease, management collaboration is needed to control both. TB-DM treatment is more difficult than TB without comorbidities, resulting in a high number of TB-DM underdiagnose findings. The purpose of this study is to know awareness, acceptance and collaboration of tuberculosis and diabetes mellitus management in Type B Hospital with DOTS in Yogyakarta City. Subjects and Methods: This study is a qualitative study with a case study research design. Research informants were 5 health workers and 3 patients selected by purposive sampling method. Data collection techniques use interviews, observation and document search. Data analysis activities in this study consist of three activities that occur simultaneously, namely data reduction, data presentation, and conclusion / verification. Results: The main themes in this study include: 1) Commitment to better results, 2) HR readiness and systems that are not optimal, 3) Good collaboration potential, 4) Readiness for collaboration. The obstacle is that there is no specific guide to TB-DM from the health institution. Supporting factors are the potential for collaboration in TB-DM treatment, and hospital readiness to perform TB-DM collaboration Conclusion: Tuberculosis and Diabetes Mellitus collaboration in Type B Hospital with DOTS in Yogyakarta City has not been fully implemented, it requires improvement of collaboration between health workers, related institution and private organizations. Awareness and acceptance about TBDM needs to be improved keywords: awareness, acceptance, kolaborasi, TB-DM