Background: The development of CAUTI bundle is very important for nurses in matters related to the urinary catheter insertion, which is one of prevention and risk control CAUTI. Purpose: To developed and implemented of catheter associated urinary tract infection (CAUTI) Bundle.. Method: This study used Mix-method method with sequential exploratory. In the qualitative stage: It used indepth interview with 16 participants and focus group discussion with 6 participants. The results of the themes analysis used content analysis that used to make the bundle CAUTI and the observation sheet. In the quantitative stage: the validity test of the bundle and the observation sheet with the validity construct with 2 experts. Validity content test with sample was 30 respondents. Continued to implementation of bundle with true experimental design that was post-test only control design. The number of sample intervention group was 32 respondents and control group was 32 respondents. Result: In qualitative results obtained 10 themes which developed into components bundle CAUTI. In quantitative results, Aiken's V validity test results ranged from 0.667-1. Result of validity test of biserial correlation, t value > t table, n = 30 (30-2 = 28), significance 5% = 2.048. Reliability of KR20 test is 0.90. Result of non-parametical test of Mann-Whitney Test, there was difference between group of intervention and control with significance 0,001 <0,05. Conclusion: Bundle development was very important as one of the prevention of CAUTI incidents in hospital. Keywords: CAUTI, Development and implementation of bundles, Validity of bundles and observation sheet.