This research was conducted at Muhammadiyah University Yogyakarta with analysis of role of locus of control variable as a variable that moderate job insecurity relationship to turnover intention. The subject of this study is all contract employees at the University of Muhammadiyah Yogyakarta, amounting to 46 respondents. This study uses SPSS 16.0 software with Moderated Regression Analysis (MRA) analysis technique. Based on the research that has been done on the contract employee at Muhammadiyah University of Yogyakarta, the result showed that job insecurity variable has a significant negative effect on turnover intention and locus of control variable can moderate by strengthening the influence of job isecurity toward turnover intention.