Hand sanitizer products on the market have harmful side effects and expensive , so it need to find an active substance which has an antiseptic hand sanitizer potency . Bee propolis is one of alternative active substance that can be used. The aim is to know effectivity Bee Propolis gel as Hand sanitizer to reduce bacterial number. Bee propolis gel hand sanitizer consist of propolis extract, carbopol-based gel and water were tested to 120 responden. Hand hygiene be done by 6 groups with alcohol, antis® (positive control), base gel (negative control), 0.3 % , 0.9 % and 15% bee propolis gel extract. Bacterial number were measure before and after hand hygiene. Bacterial number were analyzed using Wilcoxon, Kruskal-Wallis and Mann-Whitney test. The results showed The average bacterial number before hand hygiene with alcohol, Antis, Basegel, 0.3%, 0.9% and 15% bee propolis gel extract respectively are 40.25 cfu /cm2, 30.50 cfu / cm2 , 23.95 cfu / cm2 , 16.25 cfu / cm2, 41.80 cfu / cm2, 23.70 cfu / cm2, while after hand hygiene are 6.30 cfu / cm2, 8.90 cfu /cm2, 18.15 cfu /cm2, 9,40 cfu /cm2, 11,65 cfu /cm2, 4,35 cfu /cm2 Bacterial number can be reduced significantly by 0.9% and 15% bee propolis gel extract and has an equal ability with alcohol and antis® (p <0.05).