Introduction The growing debate regarding stress level of students in the clinical rotation at medical program has been receiving consirable attention recently. Medical students face numerous stressors during their clinical years, including difficult clinical events. The greater differences environments and work load compared to under-graduate contribute to increasing stress, especially when the students enter surgery clinical rotation. Our objective was to specifically studied the comparison of stress level among them when they engaged in surgery or non surgery clinical rotation. Design and Methods A cross sectional descriptive analytic study was carried out on all students in surgical fields (obsgyn, surgery) and non surgical fields (non obsgyn and non surgery) at the 3 Teaching Hospitals of Muhammadiyah University of Yogyakarta after one month discharge from their rotation. Level of stress was measured by DASS 21 after modified. The subjects consisted of 24 students in each groups. Result Data which obtained from the collection of 24 students from non-surgical group and 22 students from surgical group. Data analyzed with independent T-test. There were significant differences between surgical group compared to non-surgical group on stress level (p: 0,001, 95% CI:11,12-14,32). In surgical group found 10% students have severe depression with serious anxiety and average stress level and 5% have mild depression with average anxiety. In non-surgical group there was not found student with severe depression. Only 5% have mild depression with mild anxiety. Discussion & Conclusion Students experienced many difficult clinical events and found poor team dynamics most stressful. This is likely due to their role in the hierarchy, stress of evaluation, and vulnerable position as a medical student especially in surgical group. We believe students were not stress because of the patient care rather than the new environment and night shift everyday during ten weeks of their clinical rotation. They did not perceive medical errors or patient care events as stressful given their lack of autonomy and because they don’t yet feel responsible for patient care. Surgical group had an effect on stress level compared to non-surgical group.