Anti-emetic is a drug used to treat nausea and vomiting. The purpose of this study was to determine the effect of the compound class of essential oils Ginger (Zingiber officinale) on ileum smooth muscle contraction induced by acetylcholine agonist. The method used is in silico and in vitro, that doing molecular docking with AutoDockTools applications and experiments on smooth muscle of ileum from guinea pigs (Cavia cobaya) using organbath tools. Analysis of this form of comparative molecular docking of ligand docking original score (Tiotropium), the test compound (zingiberene) and comparator drugs (atropine). Ginger essential oil is given at a dose of 1 and 1.25 ppm, agonist were given the levels of acetylcholine series 10-8 - 10-2 M. In vitro tests have also studied the reversibility at muscarinic acetylcholine receptors. The results showed that the essential oil of ginger doses of 1 and 1.25 ppm were able to inhibit the response of guinea pig ileum smooth muscle contraction induced by acetylcholine concentration series. This was shown by a shift in the response curve isolated ileum smooth muscle contraction in the right direction with a dose-dependent pattern. Visualization docking showed that the test compound and the native ligand attached to the same residues. Ginger also has energy bond that is stronger than atropine. The conclusion of this analysis, the essential oil of ginger potential as an anti-emetic agent.