The research was conducted at PKU Muhammadiyah Bantul hospital which aims to determine the effect of work motivation, work discipline on the performance of nurses in the inpatient room. The data obtained has been analyzed using descriptive analysis and quantitative analysis. Quantitative analysis used multiple regression analysis to determine the effect of independent variables on the dependent variable, but before the test is carry out the validity and reliability has been tasted, sampling was using total sampling technique where the population is equal to the number of samples as many as 47 nurses. The results of the study revealed that work motivation factors had a significant effect on the performance of nurses in PKU Muhammadiyah Bantul hospital, while the discipline factors had a significant effect on the performance of nurses of PKU Muhammadiyah Bantul hospital. The results of the study revealed that motivation factors had a significant effect on performance as (0.049) which means that h0 was rejected and h1 was accepted. While the results of work discipline on performance had a significant effect, value of the discipline as (0.047) where the value is smaller than the standard value of error (0.05) which means h0 was rejected and h1 was accepted. From the research, it was found that the human resources at PKU Muhammadiyah Bantul hospital were still lacking in terms of motivation and in terms of work discipline on performance. It is recommended to the management of PKU Muhammadiyah Bantul Hospital District to: provide rewards for the nurse who has been performing in nursing care is good and right according to established standards and provide punishment for nurse who has not been optimal in performance. Providing training for the nurse who has never trained nursing care, especially those directly involved with the implementation of nursing care in hospital.