Kepel (Stelechocarpus burahol (Bl.) Hook F. & Th.) is one of Indonesia's native fruit plants, including rare plants. Conventional propagation of kepel plants takes a long time, propagation is done through tissue culture. Until now the kepel sterilization method has never been done. This study aims to obtain the right method of embryo and endosperm sterilization using Sodium hypochlorite (NaOCl). This research was conducted at the In vitro Culture Laboratory of the Faculty of Agriculture, Muhammadiyah University of Yogyakarta in 2018. This study was compiled using a single completely randomized design (CRD) of 8 treatments, namely immersion of explants embryonic and endosperm kepel using NaOCl in with each burn (EN5% -5', EN5% -10', EN10% -5 ', EN10% -10 ', EM5% -5', EM5% -10 ', EM10% -5' and EM10% -10 '). The results showed that embryo treatment with concentration of 10% NaOCl for 5 minutes (EM10% -5 ') was the best sterilization methode that strengthened the percentage parameters of live explants 88,89%, percentage of contamination 0% and percentage of browning 11.11%, and percentage of calli 22.22% with the largest calli diameter of 3.24 mm and color calli of white and has compact texture.