Background: Quality services are obtained from good collaboration between professions in teamwork. One effort to realize effective collaboration between professions needs to be the practice of collaboration through the learning process, namely Interprofessional Education (IPE) Purpose: This study aims to determine differences in perceptions of interprofessional competence of Universitas Muhammadiyah Yogyakarta medical students who have and have not received IPE Methods: The design of this study was pre and post test with a cross sectional analytic study design. The subjects were UMY medical students of 2015, 2016 and 2017 with a total sample of 53 students. The research instrument used a questionnaire from the Readiness for Inter Professional Learning Scale (RIPLS), which was then tested for validity and reliability by Fuziah (2010). . Data analysis using a comparative test that is paired t-test. The questionnaire distributed was 159 questionnaires. The questionnaire returned was 159 questionnaires, of which the entire questionnaire could be processed Result: The results showed that there were differences in perceptions of interprofessional competence between groups who had received IPE and had not received IPE, with a significance value showing (p = 0.004 <0.05). From the results of the analysis of the mean test, it was found that the perception for third semester students who had not received IPE was better than the 5th semester students and 7th semester who had received the IPE. This may be due to the low understanding of other professions. Students who already have experience with the practice of IPE cannot fully understand the roles of each profession. 3rd semester students have been exposed to lectures on IPE, this may be related to the perceptions of 3rd semester students. Conclution: There was a difference in perceptions of interprofessional competence between groups who had received IPE and had not received an IPE (p = 0.004 <0.05).