Background: Adolescence as a period of transition from childhood to adulthood that cause stress. The incidence of stress in adolescents in Indonesia was recorded as 11.6% in 2014. One of the efforts that can be done to overcome the stressful situation is with spiritual behaviors such as attending recitals, praying in congregation, fasting, zakat, dzikir and reading Qur’an. Research Objective: Determine the correlation between spiritual behavior and stress levels in adolescents at SMA Islam 1 Sleman. Reseach Method: This type of research is descriptive correlation. The subjects were 152 students at SMA Islam 1 Sleman. Data collection with spiritual dimension scale questionnaire and Depression Anxiety Stress Scale 42 (DASS 42). The analysis technique used with Spearman-Rho Correlation Result: The results showed that the majority of respondents were 17 years old, woman, batch 2018, did not take extracurricular activities and did not have a history of disease Conclusion: Spiritual behavior in respondent is sufficient category. Most of the stress levels in respondent are normal category. The results of the two analysis of the variables showed there is no correlation between spiritual behaviors with stress level in adolescents at SMA Islam 1 Sleman.