Background: Benzopyrene is one of the organic polycyclic aromatic hydrocarbon (PAH) compounds that comes from incomplete combustion in food ingredients as well as cigarette smoke and vehicles. Black cumin (nigella sativa) is an herbal plant that has various content of active compounds, vitamins, minerals and fatty acids, such as Thymoquinone, Fe, Na, Cu, Zn and Vitamin C. This tymoquinon has an immunomodulatory effect, which is the content that can restore imbalance immune system. Various studies have been done on black cumin seeds, namely by inducing DMBA into mice macrophages. This study aimed to determine the effect of black cumin seed oil on the benzopirene-induced phagocytic activity of macrophages Methods: The test of the effect of black cumin seed oil on the phagocytic activity of benzopirene-induced macrophages was carried out experimentally in vitro in animals tested in one way anova. This study used macrophage cells from mice that had been cultured and then divided into 8 groups: negative control, treatment group by giving black cumin seed oil with a concentration of 100 μg / ml, 50 μg / ml, 25 μg / ml, 12.5 μg / ml, 6.5 μg / ml and 3.25 μg / ml, and positive control. Phagocytic activity of peritoneal macrophages was observed by the latex method Results: Black cumin seed oil has the potential as an immunomodulator in magrophage phagocytosis activity by giving black cumin seed oil with a concentration of 6.5 μg / ml, while the potential as an immunomodulator on the phagocytic index is by giving black cumin seed oil with a concentration of 3.25 μg / ml. Conclusions: There was a significant difference between the administration of black cumin seed oil and the in vitro phagocytosis activity of the macrophages and phagocytic index induced by benzopirene.