Background: Intraocular pressure (IOP) is mainly determined by the production, circulation, and drainage of aqueous humor through the trabecular network. Parameters involved in the maintenance of IOP are aqueous flow, outflow facility, uveoscleral outflow and episcleral venous pressure. Tonometry is a diagnostic test that measures intraocular pressure. There are various types of tonometers, among others are Schiotz Tonometer and Non-Contact Tonometer (NCT). Purpose: The aim of this study was to evaluate the difference of IOP level measured by Schiotz tonometer and that measured by Non-Contact tonometer in FKIK UMY Medical Students. Research Method: Observational analysis with cross-sectional approach has been conducted. Fifty eight samples underwent examination of intraocular pressure using both Schiotz tonometer and Non-Contact tonometer. The result was analyzed using the Mann-Whitney statistical test to evaluate the significant differences in IOP measurement of both instruments. Result: The average results of IOP in right and left eye measured by Schiotz tonometer were 15,99 ± 3,45 and 16,34 ± 3,04, while by Non-Contact tonometer the average results were 10,60 ± 1,66 and 10,39 ± 1,67. There was a significant difference between IOP measured by Schiotz tonometer and Non-Contact Tonometer with p=0,000. Conclusion: There was a significant difference between IOP measured by Schiotz tonometer and by Non-Contact Tonometer. Further study related to this research is recommended.