The continuous and excessive use of pesticides in conventional paddy fields has a negative impact on the ecosystem such as the reduced diversity of natural enemies. While organic paddy field without using the application of pesticides is expected to increase the role and function of ecosystems including increased abundance and diversity of natural enemies. The study was conducted to determine the diversity, abundance of natural enemies, and potential of biological control by natural enemies in organic and conventional paddy field. A survey was conducted in Kebonagung Village, Imogiri District, Bantul, Yogyakarta in five fields of organic and conventional paddy fields. To find out the diversity and abundance of natural enemies, sweeping net and yellow sticky trap was used to collect the natural enemies at 60 day after transplanting (DAT), 70 DAT, and 80 DAT. The results showed that the diversity of natural enemies (predators and parasitoid) was not significantly between organic and conventional paddy fields. Shannon `H index on organic fields was 1.3168 and conventional was 1.3596. Meanwhile, the abundance of natural enemies (predators and parasitoid) was also not significantly different between organic land and conventional paddy fields. Simpson `S index of natural enemies on organic fields was 0.6530 and conventional was 0.6190. The potential for pest control by natural enemies in organic paddy fields in Kebonagung Village was no different than in conventional paddy fields. From this study can be concluded that the role and function of the ecosystem of organic and conventional paddy fields in Kebonagung Village can be increased by maximizing the role of natural enemies.