Introduction: Colon cancer is one of the most common causes of death in the world. This is because ofthe disease is usually only known after entering the advanced stage, which causes cancer cells to metastasize to other organs. Colon cancer WiDr is a derivative of HT-29 colon cancer cells that has a life cycle capability every 15 hours. Various studies on cancer treatment have now been carried out. One of them is by using bandotan leaf extract (Ageratum conyzoides L.). Bandotan leaves contain alkaloids, flavonoids, tannins and saponins which have anticancer effects. This study aims to determine the cytotoxic and antimigration capabilities of bandotan leaf ethanol extract (EEDB) on WiDr colon cancer cell. Method: EEDB cytotoxic activity test on WiDr colon cancer cells was performed use the MTT Test method to find out the IC50 EEDB value. Test cytotoxic is carried out in vitro to determine a cytotoxic potential compound. Furthermore, the antimigration activity of cells was observed through testing migration with the scratch wound healing assay to obtain percent closure, then analyzed using the one way ANOVA method to determine the significant differences in EEDB. Results: Bandotan leaf ethanol extract has potential as a moderate anticancer agent for WiDr with an IC50 value of 794,029 μg / ml. Giving Doxorubicin to the WiDr colon cancer cells has an IC50 value 10.64 μg / ml. Bandotan leaf ethanol extract has the potential as an antimigration agent for WiDr colon cancer cells with a final percentage of closure 4.42% by giving 1/4 IC50 of bandotan leaf ethanol extract concentration. Conclusion: There is a significant result of difference between bandotan leaf ethanol extract with cytotoxic ability and migration of WiDr colon cancer cells in vitro.