Background: Cancer is one of the disease that cause a large number of death in the world. Burkitt’s lymphoma is a type of malignancy with the fastest growth rate than others. Burkitt’s lymphoma that is happened in oral cavity can cause alveolar bone defect and tooth mobility. Treatment of Burkitt’s lymphoma using chemotherapy has side effect such as, xerostomia, mucositis, dysphagia and also bleeding. The alternative treatment by using Ant-nest plant (Myrmecodia pendens Merr.& Perry) is choosen because of minimal side effect. This plant is known as source of phytochemicals like flavonoid, tanin, saponin, triterpenoid and tokoferol, they have role as anticancer, antibacterial, antioxidant and so on. Hexane fraction of M. pendens is used to inhibit cell growth of Burkitt’s lymphoma. Objective: The research is aimed to test cytotoxic potential of hexane fraction from M. pendens against Burkitt’s lymphoma. Method: This was a purely laboratory experimental in vitro by various concentrations of hexane fraction from M. pendens, 15.625, 31.25, 62.5, 125, 250, 500 and 1000 μg/mL against Burkitt’s lymphoma which was already exposed by Eipstein-Barr virus. This cytotoxicity test used MTT Assay method to obtain viability of Burkitt’s lymphoma that is used to determine the value of IC50 of hexane fraction from M. pendens. Result: The result showed that hexane fraction of M. pendens does not show cytotoxic activity against Burkitt’s lymphoma because there was no dose-dependent phenomenon between concentration of hexane fraction and the Burkitt’s lymphoma death percentage. Conclusion: It can be concluded that hexane fraction of M. pendens does not have cytotoxic potential against Burkitt’s lymphoma in vitro.