Naive T cells and activated T cells express different adhesion molecules and patterns of migration. Naive T cells express CD62L. The research aims to assess the CD4+CD62L+ and CD8+CD62L+ profile of BALB/c mice model allergy on the sensitivity, the challenge and the gastrointestinal tract allergy phase after treatment with ethanol extract of Dioscorea alata L. (EEDA). An experimental study with post test only control group design using 63 BALB/c mice was divided into seven groups: control group (I) and treatment groups with the ethanol extract of D. alata L. tubers dose (0.00, 0.17, 2.01, 10.04) g · kg−1 bw (II-V); an antihistamine drug treatment (VI) and diosgenin treatment of 200 mg · kg−1 bw (VII). Treatment groups induced with ovalbumin i.p. on 15 d and 22 d, orally on 23 d to 30 d subsequently. Mice were sacrificed on (18, 25 and 31) d, the spleen was taken and isolated to measure CD4+CD62L+ and CD8+CD62L+ profile with flowcytometry. The results showed that EEDA dose (0.17, 10.04) g · kg−1 and drug on sensitivity phase, EEDA dose 2.01, 10.04 g · kg−1; and drug on challenge phase; EEDA dose (0.17, 2.01, 10.04) g · kg−1, antihistamine drug and diosgenin on gastrointestinal tract allergy phase increased the percentage of CD4+ expressing CD62L+, but not diosgenin on sensitivity and challenge phase, EEDA dose (0.17, 2.01, 10.04) g · kg−1, and antihistamine drug increased the percentage of CD8+ T cells expressing CD62L+ on the sensitivity, challenge, and gastrointestinal tract allergy phase. The conclusion is EEDA and antihistamine drug increase the percentage of CD4+ T cells and CD8+ T cells expressing CD62L+ on the sensitivity, challenge, and gastrointestinal tract allergy phase and diosgenin increase the percentage of CD4+ T cells and CD8+ T cells expressing CD62L+ on the gastrointestinal tract allergy phase of BALB/c mice digestive tract allergy model.