ing. Substitution of conponents in a ture medium v,ith less expensive compound such as planls e\h-act is chosen as qllettlative solutio,?. This stufi' wos aimed to deterntine the efect of Karsen (Muntingia calobura L.) act,acls os an alternative medium lor subcrlturing Dendrobium sp. The research used laboratory experiments method, i.e. single-factor treatments designs wilh 8 lreatments which arranged in completely rqalsuized design (CRD). Each lreatments was replicated 3 times, each 5 samples for each replication- Total sampels were 120 unils. The eight types ofmedium were used, i.e. VlV medium + BAP 2 ng/L + NAA I mg/L (1" contrul), leaffertilizer medium 3 g/L * coconul water 150 ml/L + banona edracl 150 g/L + sugar 30 g/I- (2tu control), Karsen exlract 50 g/L * sugar 30 g/L, Karsen extract 100 g/L + sugor 30 g/L, Karsen etract 150 g/L + sugar i0 g/L, Korsen extroct 50 g/L + sugar I 5 g/L, Karsen.edract 100 g/L + sugar I 5 g/L and Karsen 150 g/L + sugar 15 g/L. Observations were canied outfor I weeks ond the variobels observed were plant height, rutmber of leates and mrmber ofshoots. Korsen extract can be used as a substitlie medium for subculluring Dettdrobium sp. Karsen ertract concentrotion 50 g/L with the oddilion of sugar 1 5 g/L was concluded as the besl strbcultwe mediumfor Dendrobium os shown by variabels the difference of plant height i.e. 0,65 cm and the number of leaves i.e. 2,44 shoots.