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      • 04. LECTURERS ACADEMIC ACTIVITIES
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      IN VITRO STERILIZATION AND SHOOT INDUCTION OF FIG (FICUS CARICA L) USING MS CONTAINING GA3 MEDIUM SUPLLEMENTED WITH BAP AND NAA

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      Date
      2014
      Author
      RINEKSANE, INNAKA
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      Abstract
      The purpose of this research wa^s to obtain the proper sterilization method for ilg (Ficus caricaL.) shoot and determine the best combination of BAP and NAA for in vitro shoot induction offig. The sterilization research was arranged in completely randomized design (CRD), a single factor with five treatments. The treatments tested were dipping in NaCIO (0.1, 57o for 1 min), fungicide and bactericide 4 g/L (5 min, 30 min, t h, 2 h, 3 h). Each treatment was repeated three times. Parameters measured were the percentage of browning explants, the percentage of contaminated explants and the percentage of survive explants. Shoots induction research was arranged in completely randomized design (CRD), a single factor with seven treatments. The treatments tested were the addition of BAP (0, 2. 4, 6 mgll) and NAA (0, 0.5, I mg/L) to the MS medium containing GA3. Each treatment was repeated six times. Parameters measured were the percentage of contaminated explants, the percentage of survive explants, percentage of shoot formation, percentage of browning explants, number of shoots, shoot heighl, number of leaves and percentage of rooted explants. The best method for fig shoot sterilization was dipping explants in NaCIOlOTo, 5'+ NaCIO 5Vo,7' + fungicide and bactericide 4 glL for 3 h with the percentage of survival explants 100% during 30 days of observation. The addition of 2 mgll- BAP and 0.5 mgil NAA into MS containing GA3 medium was favorable to produce among the highest percentage of fig shoot fbnnation (33.33Vo) and the browning explants (O.00Ec).
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      http://repository.umy.ac.id/handle/123456789/31058
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