Background: Endodontic treatment often faces failure due to multiple causes. Bacteria resistant is one of the causes that often appear to be endodontic failure. Enterococcus faecalis is a bacterium that is frequently engaged in constant endodontic disease with a prevalence of 24% to 77%. Enterococcus faecalis has virulent factors that can harm the host cell of the setting. Enterococcus faecalis uses energy source from carbohydrate metabolism to promote the virulent bacteria activity. Propolis is one of the product of the Apis trigona that contains as antibacterial biological and pharmaceutical properties. This study aims to determine the effect of Apis trigona ethanolic propolis compound on the carbohydrate activity metabolism of Enterococcus faecalis. Propolis Apis trigona was extracted from the local apiary in the Nglipar district, Yogyakarta using a maceration procedure. Method: Enterococcus faecalis ATCC 29212 and root canal clinical sample were used for this study research. The total sample of each type of bacteria was 15. Triplicate experimental design was conducted, each of the 3 samples received therapy from the ethanolic propolis group, EEP 0.00125 percent, EEP 0.4 percent, EEP 10 percent, adverse control (BHI) and positive control (CHX). Result: Both samples have the same result, 12 samples have metabolism and 3 samples do not have metabolism, which are from positive control. Quantification of qualitative information with program image j information is statistically analyzed using Shapiro-Wilk (p>0,05), ANOVA (p<0,05) and post hoc LSD. These results imply that there are important differences between treatment groups. pH data were shown in the descriptive table Conclusion: Ethanolic propolis could not inhibit carbohydrate metabolism in Enterococcus faecalis, with the indicator changing in color from red to yellow in all EEP group therapy.