Introduction. Tissue regeneration is one of the most important goal in periodontal treatment. Carbonate Hydroxyapatite (CHA) is a synthetic scaffold that can help bone healing process after a periodontal treatment. One of the main problem that can cause a failure in periodontal surgery is bacterial reinfection. In this case, bacterial control and bacterial elimination are very important. Chlorhexidine (CHX) is a broad-spectrum antimicrobial that works as an antiseptic that effectively kills various types of microbes including bacteria, fungi and virus. The combination of CHA membranes with antimicrobial CHX is expected to reduce the reinfection of periodontal treatment caused by microbes. Therefore, research on the potential of CHA in loading and release CHX needs to be done. The aim of this study is to find out the ability of Carbonate hydroxyapatite scaffold to release antimicrobials, especially Chlorhexidine gluconate. Experimental. Membranes of Carbonate hydroxyapatite were measured using analytic balance for 10 mg each. The membranes then loaded in Chlorhexidine gluconate 0.2% for 30 minutes. 2 ml Phosphate Buffer Saline (PBS) was poured on each membrane inside the microtube to observe the release profile of CHX for 1, 2, 3 , 4, and 24 hours. Release and degradation profile were studied to observe the potential of CHA as delivery system for CHX. Result and Discussion. Data were analyzed using One Way Anova test and continued by Post Hock LSD (Least Significant Difference) test. As the result, CHX has sustained release. The amount of CHX released increased along with the length of time of release. At the same time, CHX release followed by CHA degradation. Conclusion. CHA can release the loaded CHX. At the same time, CHX release followed by CHA membrane degradation. CHA has potential as CHX delivery system.