The aim of this study was to assess nursing care practice in the hemodialysis center of PKU Muhammadiyah hospital Indonesia, in term of patient safety and infection control. Descriptive research method was employed to assess the implementation of hemodialysis procedure and managerial control in PKU Muhammadiyah hospital of Yogyakarta Indonesia. There were 30 hemodialysis patients who were purposively selected and recruited from the center. The quessionare served as the major research instrument on this study. The hemodialysis patients indicated that hemodialysis center of PKU Muhammadiyah Yogyakarta Indonesia has been prepared well for all of the aspects of safety and infection control in both procedure and managerial control. Most of nurses and health care provider are aware to provide best care for their patients along the process of care including psychological aspect such as religious practice. It is significantly important to assess nursing care practice based on the point of view of patients as the evaluation process. The result can be as data base to improve quality of nursing care.