This research to obtain the best type of medium and BAP concentration for the growth of Vanda tricolor orchid buds. The research was conducted at the In vitro Culture Laboratory of the Faculty of Agriculture, Yogyakarta Muhammadiyah University from August to October 2018. The research method used laboratory experimental methods arranged in a single Randomized Complete Design (CRD) with the treatment being tested was a combination of NDM (New Doghasima Medium), VW (Vacin and Went), MS (Murashighe and Skoog) and BAP (Benzylaminopurine) concentrations are 0, 0.5, and 1 mg / l. Each treatment was repeated 10 times, so that 90 treatment units were obtained. Each treatment was added 0.5 mg / l NAA (Napthalane Acetic Acid) and 0.2g / l active charcoal and 0.5 mℓ / PPM (plant perservative mixure). Observation parameters in this research include, the percentage of live explants, the percentage of browning explants, the percentage of explants being contaminated, the browning explant time, the explant time contaminated, the number of shoots per treatment, and the height of explant shoots. The results showed that the type of MS medium (Murashige and Skoog) and BAP (Benzylaminopurine) concentration of 0.5 mg / l was the best combination on the growth of Vanda tricolor orchid buds shown in the number of leaves and shoot height parameters.