Background : Multidrugs-Resistant Tuberculosis (MDR TB) is tuberculosis caused by drugs resistant Mycobacterium tuberculosis, at least Isoniazid and Rifampicin. MDR TB affects an efforts to tuberculosis cases eradication in the world. In 2014, 153 countries had reported MDR TB cases. Drug resistance can be due to indisciplined previous TB treatment, such as relapse, failure, and drop out. Purpose : The purpose of this study is to determine the relationship between Tuberculosis treatment history with the incidence of Multidrugs-Resistant Tuberculosis (MDR TB). Methods : This research uses cross sectional studies in tuberculosis patients at dr. Ario Wirawan Hospital of Lung Salatiga examined by Gene Xpert MTB/RIF examination and obtaining Anti-tuberculosis medication therapy with a complete medical record about his tuberculosis treatment history. Samples of 88 tuberculosis patients were obtained by purposive sampling methods and data tracing through the patient's medical record. Results : Eighty-eight patients diagnosed with TB showed 43 (48.8%) aged 26-45 years, 50 (56.8%) are men. History of previous drug treatment TB was 38 (43.2%) relapse, 13 (14.8%) failed, 11 (12.5%) patients end the drug and 26 (29.5%) new TB patients. Gene Xpert's examination results were 53 (60.2%) TB Rifampicin resistant and 35 (39.7%) TB Rifampicin sensitive. Cross-tabulation results show the subject of research dominated by 25 (28.4%) people with a history category of relapse treatment and RIF resistant status. The Chi-Square test obtained a value of P = 0.001 (P < 0.05). Spearman test obtained the value of P = 0.033 (P < 0.05), Value of R = 0.228 with the direction of correlation is positive (+). Conclusion : It can be concluded that the history of tuberculosis treatment is associated with Multidrugs-Resistant Tuberculosis (MDR TB) incidence