| dc.contributor.author | HIDAYATI, TITIEK | |
| dc.contributor.author | AKROM, AKROM | |
| dc.contributor.author | INDRAYANTI, INDRAYANTI | |
| dc.contributor.author | SAGIRAN, SAGIRAN | |
| dc.date.accessioned | 2020-05-28T03:46:25Z | |
| dc.date.available | 2020-05-28T03:46:25Z | |
| dc.date.issued | 2019-08 | |
| dc.identifier.citation | Harvard | en_US |
| dc.identifier.issn | 2278-4527 | |
| dc.identifier.issn | 0972-0626 | |
| dc.identifier.uri | http://repository.umy.ac.id/handle/123456789/34304 | |
| dc.description.abstract | This study was conducted to investigate the
chemopreventive effect of black cumin seeds oil
(Nigella sativa) in 96 dimethylbenzanthracene
(DMBA)-induced Sprague Dawley mice. The test
animals were divided into eight groups. The normal
group was given standard food and drink. The three
black cumin seed oil (BCSO) treatment groups
received BCSO dosages of 0.25, 2.5 and 5
ml/kgBW/day respectively and they were induced with
DMBA. The thymoquinone and tamoxifen groups
received 50 mg/kgBW/day thymoquinone and 0.6
mg/kgBW/day tamoxifen respectively and they were
induced with DMBA.
The DMBA group was induced with 10x20mg/kgBW
DMBA for five weeks. The solvent group received
standard feeding and corn oil solutions. Starting from
the third week of treatment, all groups except the
normal and solvent groups were given 20 mg/kgBW
DMBA twice a week for five weeks. Dissection and data
retrieval were conducted at week 27. The
chemopreventive effects are measured by nodules
incidence, nodul multiplication, total weight and
histopathology.
The p53 and H-Ras gene expression are assessed using
densitometry after PCR. The mean intergroup
difference was calculated using one-way ANOVA. The
results showed that BCSO administration before and
during DMBA induction could decrease nodule
formation and count, decrease H-Ras gene expression
and increase p53 gene expression. A dose of 0.25
ml/kgBW/day BCSO indicated a chemopreventive
effect, increased p53 gene expression and decreased H-
Ras gene expression, all of which were similar to a dose
of 2.25 ml/kgBW/day but safer. It can be concluded that
treatment of 0.25 ml/kgBW/day BCSO produces
chemopreventive effects in DMBA-induced SD rats. | en_US |
| dc.description.sponsorship | Tchnology research and higher education ministries | en_US |
| dc.language.iso | en | en_US |
| dc.publisher | World Researchers Associations | en_US |
| dc.relation.ispartofseries | 23;8 | |
| dc.subject | Chemopreventive | en_US |
| dc.subject | Thymoquinone | en_US |
| dc.subject | Black Cumin Seed Oil | en_US |
| dc.subject | p53 gene | en_US |
| dc.subject | H-Ras Gene | en_US |
| dc.title | Chemopreventive effect of black cumin seed oil (BCSO) by increasing p53 expression in dimethylbenzanthracene (DMBA)-induced Sprague Dawley rats | en_US |
| dc.type | Article | en_US |
