Background : Chronic Renal Failure (CRF) is a serious global public health problem that requires hemodialysis as a therapy for replacing the functions of the kidney. Decreases in erythropoietin production due to kidney damage can cause anemia and it requires EPO supply therapy. This study aims to determine the relation in EPO therapy frequency towards the iron status of chronic kidney failure patients who undergo hemodialysis. Method : This research used an analytic observational research method through a cross-sectional approach with correlation test analysis. The number of samples in this research were 30 patient medical records. The independent variable of research was the EPO supply frequency and the dependent variable was the level of iron status. After the data was obtained, then it was analyzed by the Spearman correlation test. Result : The results showed that the patient with chronic kidney disease distributed into Fe levels normal 20% and decreased by 80%. Whereas the TIBC level increased by 6.7%, normal by 43.3%, and decreased by 50%. Furthermore, the SAT level increased by 6.7%, normal by 23.3%, and decreased by 70%. The analysis results of Spearman test obtained significance value p = 0.020 to Fe, significance value p = 0.257 to TIBC, and significance value p = 0.010 to SAT. Conclusion : Based on these results, it can be concluded that there are significant correlations between the frequency of EPO therapy with Fe, SAT but not with TIBC.