Two-phase flow is part of the multi-phase flow. It can occur in large, small, or mini pipes. Mini pipe two-phase flow can be applied to high-density multi-chip modules on supercomputers, X-ray equipment, high-flux heat exchangers in aerospace systems, cryogenic cooling systems on satellites, and other high-power diagnostic equipment. The more widespread application of the two-phase flow on mini pipes, so it is very needed to study its problem there. One crucial issue is increasing pressure suddenly caused by a certain flow pattern. The research was carried out using experimental methods, with an inner glass pipe diameter of 1.6 mm and a length of 130 mm. The test section with a slope of 5 degrees against the horizontal position. The visualization method used a high-speed camera. The working fluid being used were air and water mixed with Glycerine with concentrations of 40%, 50%, 60%, and 70% for each blend, and varying the superficial gas and water velocity in the range JG = 0.207 m/s–66.3 m/s; JL = 0.149 m/s–4.238 m/s. The results of the videos obtained were then analyzed based on the values of JG and JL. Furthermore, they were plotted in the flow pattern maps. This research was divided into five new flow patterns: i.e., mini bubble, long plug, slug, slug-annular, and churn. The flow patterns were then compared to the previous research and indicated conformity but there was a shift in the transition line. This research also found a new invention that the slug flow pattern affected the increasing pressure difference significantly.