Abstract — Background : Hyperglycemia in Diabetes Mellitus will increase the activity of NADPH oxidase, which can further increase oxidative stress in the body and MDA as an indicator of lipid peroxidation. This study aims to determine the effect of hyperglycemia on MDA levels as a predictor of oxidative stress in the rat model of a diabetic. Method : It is an experimental study using male Wistar rats, 8-10-week-old, bodyweight of 200-250 grams. Diabetic STZ induction used (streptozocin) injection 45 mg/kg BW and NA (Nicotinamide) 110 mg/kg BW . Three days after induction, blood glucose level increaseD (> 250 mg/dl) in a diabetic state. Blood Glucose Level and MDA level were examined in H-0, H-7, H-14, H-21, and H-28 each group using 3 rats. Data were analyzed using one-way ANOVA and correlation with SPSS. Result S And Analysys : The results of this study showed that there was an increase of Blood Glucose Level and MDA level on H-0, H-7, H-14, H-21, and H-28. The peak of Blood Glucose Level on H-14 was stable but remained high. MDA level also increased, and the peak level was on H-28. Furthermore, there was significant increases in Blood Glucose level and MDA (p = 0.001). Correlation analysis showed that there is a strong correlation between Blood Glucose Level and MDA level (r = 0.728). Conclusion : Hyperglycemia causes an increase in oxidative stress in the rat model of diabetic