Introduction Ventilator associated pneumonia (VAP) is a nosocomial infection in patients using mechanical ventilation. The prevalence of VAP as much as 9-27% with a mortality rate of ≥ 50%. VAP-causing microorganisms are Staphylococcus aureus, Pseudomonas aeruginosa and Enterobacteriacea. Each hospital need susceptibility pattern for empirical antibiotic treatment of VAP. Objective To know the susceptibility pattern and empirical antibiotic treatment on Ventilator Associated Pneumonia (VAP) in PKU Muhammadiyah Hospital Yogyakarta in 2011-2014. Methode Descriptive observational retrospective study with cross sectional approach. Sample sputum from all inpatients diagnosed with VAP within January 2011 until October 2014, according to the medical record. Results: Sixty-five VAP patients 55.38% are males and 50.77% are 51-75 years old. There are four most common microorganisms isolated from sputum samples : Pseudomonas aeruginosa (15.38%), Staphylococcus aureus (13.85%), Candida albicans (9.23%) and Klebsiella pneumoniae (7.69%) The susceptibility pattern isolate from sputum sample of VAP are Amikacin the most sensitive (53.85%) and the lowest sensitive are Cefotaxime and Minocycline (1.54%). There are four most common empirical antibiotic treatment were given by clinicians : Ceftazidim (35.28%), Levofloxacin (29.23%), Ceftriaxon (21.54%) and Cefixime (10.77%). Conclusion : Pseudomonas aeruginosa is the most common cause of VAP infection in PKU Muhammadiyah Hospital Yogyakarta, while Amikacin showed the highest sensitivity percentage and the most common choice of empirical antibiotic treatment by clinicians is Ceftazidim.